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Keywords from Image Description:
Ligase rxn. en Enzymatic steps of DNA ligation by DNA ligase Lig First enzyme AMP is formed by the attack of Lys on the alphaphosphate of ATP or NAD releasing inorganic pyrophosphate PPi or nicotinamide mononucleotide NMN Next the 'phosphate ' of the nicked DNA strand attacks the Lys AMP intermediate to form an AppDNA intermediate